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Oxford Instruments
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MathWorks Inc
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SourceForge net
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Siemens AG
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Siemens AG
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Siemens AG
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Image Search Results
Journal: Thrombosis and Haemostasis
Article Title: miR-204-5p and Platelet Function Regulation: Insight into a Mechanism Mediated by CDC42 and GPIIbIIIa
doi: 10.1055/a-1497-9649
Figure Lengend Snippet: Effect of miR-204-5p on megakaryocyte differentiation. Fluorescence-activated cell sorting analysis at D15 showed that miR-204-5p ( A ) had no effect on megakaryocyte ploidy, and ( B ) no effect on the expression of the specific differentiation markers CD41, CD42b, and CD42d. miR-204-5p decreased megakaryocyte polarization defined as the colocalization of F-actin and GP1bβ ( C and D : images obtained using an LSM700 microscope, background subtraction, and application of a Gaussian filter using Imaris 9.5.1 software for 3D rendering; scale bars = 10 μm). n = 3 to 5 independent experiments; * p < 0.05, *** p < 0.001.
Article Snippet: The three-dimensional reconstitution of z-stacks was performed using a background subtraction and the application of a
Techniques: Fluorescence, FACS, Expressing, Microscopy, Software